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A bacterium having CMCase activity was isolated form soil and identifed as a Pseudomonas sp YD-15. The optimum conditions for the production of CMCase were avicel 1.2%, yeast extract 0.5%, KNO₃ 0.06%, K₂HPO₄ 0.2%, MgSO₄·7H₂O 0.15%, pH 8.0, 30℃ and 60 hours cultivation. The CMCase was purified 15.3 folds with 14% yield through ammonium sulfate precipitation, DEAE-sepharose column chromatography and sephadex G-100 gel filtration chromatography. The optimum pH and temperature for the enzyme activity were 6.0 and 50℃, respectively. The enzyme was stable between pH 5.0 and 8.0, below 50℃. The molecular weight was calculated about 100,000 by SDS-polyacrylamide gel electrophoresis. K_m value for CMC used as a substrate was 40 ㎎/㎖.
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